Search results for " European corn borer"
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Study of two midgut aminopeptidases from Ostrinia nubilalis Hubner
2008
Aminopeptidases N (APNs) have been identified as Bacillus thuringiensis endotoxins receptor candidates in several Lepidopteran species. Employing the RACE PCR technique we obtained two complete cDNAs corresponding to two APNs expressed in the midgut of Ostrinia nubilalis larvae. One of the sequences was 3624 bp long, and the predicted protein was composed by 940 aminoacids, whereas the other cDNA was 3226 nucleotides long, leading a putative protein composed by 994 aminoacids. The in silico study of the sequences, showed in both proteins a signal peptide, a GPI-anchor domain, a zinc-binding region HEXXHX18E and a GAMEN motif, characteristic of the gluzincin aminopeptidases. Moreover, severa…
Susceptibility of ostrinia nubilalis to bacillus thuringiensis var. kurstaki
2003
Baseline susceptibility of European corn borer larvae, Ostrinia nubilalis (Hübner), to a commercial formulation of Bacillus thuringiensis var. kurstaki was determined for different strains in 7 geographic locations in the corn area of North Italy (Po Valley). For the bioassay 7 different doses, raised on a meredic diet, were used and the data was analysed with the Probit analysis. Differences in the susceptibility among the northern Italian populations of O. nubilalis were recorded, which can be attributed to natural variability. The ECB collected near Como and Torino was the most susceptible, respectively with LC50 of 0.23 and 0.57 mg/ml. The most tolerant ones were the larvae coming from …